| Autor: |
Steimle, Alex, De Sciscio, Alessandro, Neumann, Mareike, Grant, Erica T., Pereira, Gabriel V., Ohno, Hiroshi, Martens, Eric C., Desai, Mahesh S. |
| Zdroj: |
STAR Protocols; June 2021, Vol. 2 Issue: 2 |
| Abstrakt: |
Reproducible in vivomodels are necessary to address functional aspects of the gut microbiome in various diseases. Here, we present a gnotobiotic mouse model that allows for the investigation of specific microbial functions within the microbiome. We describe how to culture 14 different well-characterized human gut species and how to verify their proper colonization in germ-free mice. This protocol can be modified to add or remove certain species of interest to investigate microbial mechanistic details in various disease models. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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