| Autor: |
Anand, KB, Agrawal, P, Kumar, S, Kapila, K |
| Zdroj: |
Indian Journal of Medical Microbiology; January-March 2009, Vol. 27 Issue: 1 p27-29, 3p |
| Abstrakt: |
Cefoxitin is a potent inducer of the mecAregulatory system. It is being recommended for detection of methicillin resistance in Staphylococcus aureus(MRSA) when using disk diffusion testing. The aim of our study was to evaluate the efficacy of cefoxitin disc diffusion test to characterize MRSA and compare it with oxacillin agar screening and detection of mecAgene by PCR. Materials and Methods:Fifty strains of S. aureusisolated from clinical samples were used in the study. Routine antibiotic susceptibility testing was performed including oxacillin disk. Oxacillin screen agar plates with 4% NaCl and 6 µg/ml of oxacillin were inoculated and interpreted as per standard guidelines. Cefoxitin disc diffusion test was performed using 30 µg disc and zone sizes were measured. PCR for amplification of the mecAgene was performed. Results:Out of the 50 isolates, 28 were found to be methicillin resistant by oxacillin disc diffusion test, 30 were resistant by oxacillin screen agar method, and 32 were resistant with cefoxitin disc diffusion. For these 32 isolates mecAgene was positive. Conclusion:Results of cefoxitin disc diffusion test is in concordance with the PCR for mecAgene. Thus, the test can be an alternative to PCR for detection of MRSA in resource constraint settings. |
| Databáze: |
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