| Autor: |
Cohen, Pascale, Bouaboula, Monsif, Bellis, Michel, Baron, Véronique, Jbilo, Omar, Poinot-Chazel, Caroline, Galiègue, Sylvaine, Hadibi, El-Hadi, Casellas, Pierre |
| Zdroj: |
Journal of Biological Chemistry; April 2000, Vol. 275 Issue: 15 p11181-11190, 10p |
| Abstrakt: |
Listeria monocytogenesis a pathogenic intracellular microorganism whose infection induces pleiotropic biological changes associated with host cell gene expression regulation. Here we define the gene expression profiles of the human promyelocytic THP1 cell line before and after L. monocytogenesinfection. Gene expression was measured on a large scale via oligonucleotide microarrays with probe sets corresponding to 6,800 human genes. We assessed and discussed the reproducibility of the hybridization signatures. In addition to oligonucleotide arrays, we also performed the large scale gene expression measurement with two high-density membranes, assaying for 588 and 18,376 human genes, respectively. This work allowed the reproducible identification of 74 up-regulated RNAs and 23 down-regulated RNAs as a consequence ofL. monocytogenesinfection of THP1. The reliability of these data was reinforced by performing independent infections. Some of these detected RNAs were consistent with previous results, while some newly identified RNAs encode gene products that may play key roles inL. monocytogenesinfection. These findings will undoubtedly enhance the understanding of L. monocytogenesmolecular physiology and may help identify new therapeutic targets. |
| Databáze: |
Supplemental Index |
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