| Autor: |
Lloyd-Jones, John G., Heidel, Peter, Yagen, Boris, Doyle, Phillip J., Friedell, G.H., Caspi, Eliahu |
| Zdroj: |
Journal of Biological Chemistry; October 1972, Vol. 247 Issue: 19 p6347-6354, 8p |
| Abstrakt: |
Evidence is presented for the in vivoincorporation of 3H and 14C into cholesterol and 5α-cholest-7-en-3β-ol following the administration of l-[methyl-3H, 14C]methionine to normal and mammary tumor-bearing rats. The incorporation of the 14C into the total crude nonsaponifiable residue was of the order of 0.05% of the administered 14C. In all experiments the 3H:14C ratio of the cholesterol was 3 to 4 times higher than that of the 5α-cholest-7-en-3β-ol isolated from the same animals. An enrichment of tritium in the cholesterol isolated from normal animals was noted. Thus, while the cholesterol isolated from tumor-bearing animals had a 3H:14C ratio similar to that of the administered methionine, the 3H:14C ratio of the cholesterol from normal rats was about 50% higher. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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