| Autor: |
Smith, Christopher J., Teh, Hung-Sia, Ley, Arthur N., D'Obrenan, Perry |
| Zdroj: |
Journal of Biological Chemistry; June 1973, Vol. 248 Issue: 12 p4475-4485, 11p |
| Abstrakt: |
A major and a minor lysine-specific transfer ribonucleic acid (tRNA) were purified from the haploid yeast Saccharomyces cerevisiaeαS288C by column chromatography on benzoylated diethylaminoethylcellulose. The nucleotide sequence of the major lysine tRNA was determined by use of 32P-labeled material. The molecule is 76 nucleotide residues in length and contains the anticodon C-U-U. In experiments to determine coding specificity, the major lysine tRNA showed the greatest response with the codon A-A-G. Preliminary studies on the structure of the minor lysine tRNA showed that its structure is most probably identical with one of the two major lysine tRNAs from bakers' yeast. The minor lysine tRNA recognizes both the lysine codons A-A-G and A-A-A weakly. The anticodon proposed for bakers' yeast lysine tRNA is S-U-U, where Sis the 3′-phosphate of 2-thio-5-carboxymethyluridine. The major lysine tRNA differs from bakers' yeast lysine tRNA in a total of 21 nucleotides. Only the G-T-ψ-C loop and its supporting stem are identical. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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