Amidation and Cross-Linking of the Enzymatically Synthesized Peptidoglycan of Bacillus stearothermophilus

Autor: Linnett, Paul E., Strominger, Jack L.
Zdroj: Journal of Biological Chemistry; April 1974, Vol. 249 Issue: 8 p2489-2496, 8p
Abstrakt: A particulate enzyme system from Bacillus stearothermophiluswhich catalyzes the synthesis of peptidoglycan from uridine nucleotide precursors has been investigated. The preparation catalyzed extensive cross-linking of the enzymatically synthesized peptidoglycan. In addition in the presence of ATP and ammonia or l-glutamine amidation of the peptidoglycan also occurred. The nature of the amidated products obtained on lysozyme digestion of the enzymatically synthesized peptidoglycan was elucidated by a comparison with similar materials obtained after lysozyme digestion of isolated vegetative cell walls of this organism.
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