| Autor: |
Tkacz, Jan S., Herscovics, Annette, Warren, Christopher D., Jeanloz, Roger W. |
| Zdroj: |
Journal of Biological Chemistry; October 1974, Vol. 249 Issue: 20 p6372-6381, 10p |
| Abstrakt: |
Calf pancreas microsomes incorporated radioactive mannose from GDP-d-[14C]mannose into products precipitated by 6 % trichloroacetic acid-0.5 % phosphotungstic acid. This incorporation was maximally stimulated by 10 mmMn2+ions and showed a broad pH optimum of 6.0 to 6.7 in Tris-maleate buffer. Between 70 and 90 % of the radioactivity was found in products soluble in chloroform-methanol (2:1, v/v). The rates of labeling of the chloroform-methanol extract and of the residual precipitate were constant for 10 and 30 min, respectively. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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