| Autor: |
Nagayama, Shin, Tojima, Takuro, Morimoto, Mayumi, Sasaki, Shigeo, Kawabata, Kazushige, Ushiki, Tatsuo, Abe, Kazuhiro, Etsuro Ito, Etsuro Ito |
| Zdroj: |
Japanese Journal of Applied Physics; June 1997, Vol. 36 Issue: 6 p3877-3877, 1p |
| Abstrakt: |
We examined the practical scan speed for the observation of live neurons in a physiological solution using atomic force microscopy (AFM) with a desired vertical resolution of the order of 10-8m, which was reasonable when taking into account that a flicker of extracellular protein and saccharide on the neurons in the solution occurred during an observation period of a couple of minutes. The practical scan speed was found to be under 40 µ m/s, therefore, if we applied AFM using 100 lines and 100 pixels per line to an observation area of 20 µ m×20 µ m, the minimum period for acquiring one image was estimated to be about 2 min. This procedure gave us good images that represented the slow three-dimensional dynamics in live neurons, such as the retrograde movement of surface protuberances, but suggested that another approach was required to detect fast structural changes induced by stimulation. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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