| Autor: |
Amore, Alessandro, Cappelli, Giorgio, Cirina, Paola, Conti, Giovanni, Gambaruto, Caterina, Silvestro, Leandra, Coppo, Rosanna |
| Zdroj: |
Nephrology Dialysis Transplantation; April 2003, Vol. 18 Issue: 4 p677-688, 12p |
| Abstrakt: |
The heat sterilization of glucose solutions for peritoneal dialysis (PDS) induces the formation of glucose degradation products (GDPs), a phenomenon amplified by lactate and neutral pH. In the new three-compartment bag (3CB) PDS, a glucose solution at pH 3 is kept apart from the buffer until use, and the final solution delivers glucose concentrations that are similar to traditional PDS (TPD), with pH 6 and a lower content of GDPs. As GDPs have oxidant activity that may favour apoptosis, we investigated mesothelial cell apoptosis modulation by 12 h cultures in media supplemented with: (i) two relevant GDPs, methylglyoxal (MGly) and formaldehyde (For) in time and dose-dependence assays, (ii) GDPs at concentrations detected in TPD and 3CB, and (iii) commercial TPD and 3CB PDS, both with 1.36% glucose. |
| Databáze: |
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