| Autor: |
Auboiron, Morgane, Vasseur, Pauline, Radman-Livaja, Marta |
| Zdroj: |
STAR Protocols; June 2021, Vol. 2 Issue: 2 |
| Abstrakt: |
Tracking the inheritance patterns of proteins (TrIPP) is a live-cell imaging technique used for tracking maternal protein segregation patterns between mother and daughter cells during asymmetric divisions of budding yeast. We use the photo-convertible fluorescent protein Dendra2 fused to a protein of interest (POI). Irreversible conversion from green to red fluorescence allows for parallel monitoring of old and new proteins for several generations. Single-cell quantitative image analysis of time-lapse microscopy gives synthesis and decay rates, as well as segregation patterns of the POI. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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