Localization of the membrane perturber adamantyl sulfate in sarcoplasmic reticular vesicles
| Autor: | Morse, Philip D. |
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| Zdroj: | Chemistry and Physics of Lipids; December 1977, Vol. 20 Issue: 4 p295-303, 9p |
| Abstrakt: | The properties of a new membrane perturbing agent, adamantyl sulfate (A-1-S) are described. Lobster sarcoplasmic reticular vesicles (SRV) were used as a test membrane system and the activity of the Ca2+-Mg2+-dependent ATPase in conjunction with spin label data was used to determine the location of A-1-S in the membrane. The results suggest that A-1-S perturbs only the polar region of the SRV and that this causes loss of ATPase function. Thus, ATPase activity requires integrity of the membrane polar region. |
| Databáze: | Supplemental Index |
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