| Autor: |
Furubayashi, Maiko, Kubo, Akiko, Takemura, Miho, Otani, Yuko, Maoka, Takashi, Terada, Yoshinobu, Yaoi, Katsuro, Ohdan, Kohji, Misawa, Norihiko, Mitani, Yasuo |
| Zdroj: |
Journal of Agricultural and Food Chemistry; May 2021, Vol. 69 Issue: 17 p5076-5085, 10p |
| Abstrakt: |
Capsanthin, a characteristic red carotenoid found in the fruits of red pepper (Capsicum annuum), is widely consumed as a food and a functional coloring additive. An enzyme catalyzing capsanthin synthesis was identified as capsanthin/capsorubin synthase (CCS) in the 1990s, but no microbial production of capsanthin has been reported. We report here the first successful attempt to biosynthesize capsanthin in Escherichia coliby carotenoid-pathway engineering. Our initial attempt to coexpress eight enzyme genes required for capsanthin biosynthesis did not detect the desired product. The dual activity of CCS as a lycopene β-cyclase as well as a capsanthin/capsorubin synthase likely complicated the task. We demonstrated that a particularly high expression level of the CCSgene and the minimization of byproducts by regulating the seven upstream carotenogenic genes were crucial for capsanthin formation in E. coli. Our results provide a platform for further study of CCS activity and capsanthin production in microorganisms. |
| Databáze: |
Supplemental Index |
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