| Autor: |
Böhnlein, E, Ballard, D W, Bogerd, H, Peffer, N J, Lowenthal, J W, Greene, W C |
| Zdroj: |
Journal of Biological Chemistry; May 1989, Vol. 264 Issue: 15 p8475-8478, 4p |
| Abstrakt: |
T cell mitogens induce the expression of specific trans-acting DNA binding proteins that in turn regulate the expression of the interleukin-2 receptor-α (IL-2Rα) gene. To investigate whether de novoprotein synthesis is required for the activation of these transacting factors and the induced expression of this receptor gene, Jurkat T cells were incubated with various inhibitors of protein synthesis prior to stimulation with phytohemagglutinin and phorbol 12-myristate 13-acetate (PMA). Despite the presence of cycloheximide or anisomycin at concentrations sufficient to block > 97% of cellular protein synthesis, phytohemagglutinin and phorbol 12-myristate 13-acetate effectively induced the expression of the IL-2R α gene as measured at the mRNA level. Similarly, gel retardation, DNA footprinting, and DNA-protein cross-linking studies revealed that these mitogens induced the activation of two predominant DNA binding proteins (50–55 and 80–90 kDa) in the presence or absence of cycloheximide and anisomycin. Both of these proteins specifically interacted with a κB-like binding site present in the IL-2Rα promoter (−267 to −256) that is requisite for mitogen-induced expression of this receptor gene. These findings support a post-translational mechanism of induction of pre-existing, but inactive, DNA binding proteins which in turn bind to and activate the IL-2Rα gene. |
| Databáze: |
Supplemental Index |
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