| Autor: |
Gan, T E, Brumley, J L, Van der Weyden, M B |
| Zdroj: |
Journal of Biological Chemistry; June 1983, Vol. 258 Issue: 11 p7000-7004, 5p |
| Abstrakt: |
Cytosolic thymidine kinase (EC 2.7.1.21) has been purified 5200-fold to apparent homogeneity from normal human placenta. The purification includes sequential affinity chromatography on blue-Sepharose and a thymidine column. The molecular weight of the enzyme determined by gel filtration and sucrose density ultracentrifugation is 92,000. The subunit molecular weight is 44,000, suggesting that the enzyme is a dimer in its native state. With isoelectric focusing, placental thymidine kinase demonstrated a single form with an isoelectric point of 9.1. The final purified enzyme preparation exhibits no immunological cross-reactivity with human mitochondrial thymidine kinase. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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