| Abstrakt: |
In an earlier study a mutant Dictyosteliumcell-line (plc−) was constructed in which all phospholipase C activity was disrupted and nonfunctional, yet these cells had nearly normal Ins(1,4,5)P3levels (Drayer, A. L., Van Der Kaay, J., Mayr, G. W, Van Haastert, P. J. M. (1990) EMBO J.13, 1601-1609). We have now investigated if these cells have a phospholipase C-independent de novopathway of Ins(1,4,5)P3synthesis. We found that homogenates of plc−cells produce Ins(1,4,5)P3from endogenous precursors. The enzyme activities that performed these reactions were located in the particulate cell fraction, whereas the endogenous substrate was soluble and could be degraded by phytase. We tested various potential inositol polyphosphate precursors and found that the most efficient were Ins(1,3,4,5,6)P5, Ins(1,3,4,5)P4, and Ins(1,4,5,6)P4. The utilization of Ins(1,3,4,5,6)P5, which can be formed independently of phospholipase C by direct phosphorylation of inositol (Stephens, L. R. and Irvine, R. F.(1990) Nature 346, 580-582), provides Dictyosteliumwith an alternative and novel pathway of de novoIns(1,4,5)P3synthesis. We further discovered that Ins(1,3,4,5,6)P5was converted to Ins(1,4,5)P3via both Ins(1,3,4,5)P4and Ins(1,4,5,6)P4. In the absence of calcium no Ins(1,4,5)P3formation could be observed; half-maximal activity was observed at low micromolar calcium concentrations. These reaction steps could also be performed by a single enzyme purified from rat liver, namely, the multiple inositol polyphosphate phosphatase. These data indicate that organisms as diverse as rat and Dictyosteliumpossess enzyme activities capable of synthesizing the second messengers Ins(1,4,5)P3and Ins(1, 3, 4, 5)P4via a novel phospholipase C-independent pathway. |
