| Autor: |
Culotta, Valeria Cizewski, Klomp, Leo W.J., Strain, Jeffrey, Casareno, Ruby Leah B., Krems, Bernhard, Gitlin, Jonathan D. |
| Zdroj: |
Journal of Biological Chemistry; September 1997, Vol. 272 Issue: 38 p23469-23472, 4p |
| Abstrakt: |
Copper is distributed to distinct localizations in the cell through diverse pathways. We demonstrate here that the delivery of copper to copper/zinc superoxide dismutase (SOD1) is mediated through a soluble factor identified as Saccharomyces cerevisiaeLYS7 and human CCS (copperchaperone for SOD). This factor is specific for SOD1 and does not deliver copper to proteins in the mitochondria, nucleus, or secretory pathway. Yeast cells containing alys7Δnull mutation have normal levels of SOD1 protein, but fail to incorporate copper into SOD1, which is therefore devoid of superoxide scavenging activity. LYS7 and CCS specifically restore the biosynthesis of holoSOD1 in vivo. Elucidation of the CCS copper delivery pathway may permit development of novel therapeutic approaches to human diseases that involve SOD1, including amyotrophic lateral sclerosis. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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