| Autor: |
Blank, R S, McQuinn, T C, Yin, K C, Thompson, M M, Takeyasu, K, Schwartz, R J, Owens, G K |
| Zdroj: |
Journal of Biological Chemistry; January 1992, Vol. 267 Issue: 2 p984-989, 6p |
| Abstrakt: |
To assess the role of cis-acting elements within the smooth muscle alpha-actin gene in smooth muscle cells (SMC), we transfected chicken smooth muscle alpha-actin promoter-chloramphenicol acetyltransferase gene fusion plasmids into SMC derived from rat and chicken aortas. In marked contrast to effects in chicken skeletal myoblasts and fibroblasts, p122CAT (positions −122 to +19), containing two conserved CArG elements, elicited a modest increase in chloramphenicol acetyltransferase reporter activity in chicken SMC. Addition of upstream sequences between −122 and −151 (p151CAT) increased activity in adult chicken SMC. Addition of sequence between positions −151 and −257 (p257CAT) resulted in a 7-fold increase in chloramphenicol acetyltransferase activity over that of p151CAT in rat SMC, but not in chicken SMC. A genomic clone encoding the rat smooth muscle alpha-actin gene was isolated, and the 5'-flanking region was partially characterized. Comparison of primary sequence between rat and chicken promoters showed a conserved E box motif at position −214 in the chicken gene and at position −213 in the rat gene. Results of these studies demonstrate that regions upstream of the conserved CArG elements exert potent regulatory effects on transcription and that SMC require different cis-acting elements than other cell types to transcriptionally regulate this gene. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
