| Autor: |
Crouse, G F, Simonsen, C C, McEwan, R N, Schimke, R T |
| Zdroj: |
Journal of Biological Chemistry; July 1982, Vol. 257 Issue: 13 p7887-7897, 11p |
| Abstrakt: |
We constructed a gene library from a murine cell line with amplified dihydrofolate reductase (dhfr) genes by inserting random segments of DNA into lambda Ch4A. From this library, the dhfr gene and 30 kilobase pairs of surrounding DNA were cloned, and the restriction map was determined. All of the coding regions were sequenced and show that the gene spans a total of 31 kilobase pairs and has five intervening sequences in the coding portion of the gene. In addition, two classes of variant dhfr genes were found in the amplified line, which were amplified and present at levels of 10 to 30% of the normal dhfr genes. Numerous repeated sequences were located throughout the gene region, some of which share homology with previously defied families of repeats. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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