| Autor: |
Le, Mai Huong, Do, Hai Doan, Tran Thi, Hong Ha, Dung, Le Vu, Nguyen, Hoai Nam, Tran Thi, Hang Nhu, Nguyen, Luyen Dinh, Hoang, Chi Kim, Le, Huu Cuong, Le Thi, Thu Huong, Trinh, Hoang Trung, Ha, Phuong Thu |
| Zdroj: |
Advances in Natural Sciences: Nanoscience and Nanotechnology; December 2016, Vol. 7 Issue: 4 p045019-045019, 1p |
| Abstrakt: |
Curcumin is a polyphenol from turmeric Curcuma longaL that has been proved to possess numerous biological and pharmaceutical activities, including anti-cancer properties. However, curcumin has only limited clinical applications due to the aqueous insolubility characteristic that reduces its biological availability. On the other hand, using nanoparticles as drug delivery system has potential as it increases solubility of hydrophobic substances such as curcumin. Furthermore, nanoparticles can protect and control release of drug. Therefore, the objective of this project is to prepare nanoparticles by polymeric encapsulating curcumin by 1-3/1-6 ?-glucan extracted from Vietnamese mushrooms to increase drug delivery efficiency and biological effect. Method of the preparation is nano-precipitation. The produced curcumin-?-glucan-nanoparticles (NanoGluCur) takes spherical shape with 60-70 nm in diameter. As expected, water solubility of curcumin increases about 180 times, from 0.6 ?g ml?1to 0.11 mg ml?1. Loading capacity of NanoGluCur is 18.16%. In vitrocytotoxicity and anti-tumor promoting effects of NanoGluCur were also investigated. Results revealed that NanoGluCur is able to inhibit the growth of two human cancer cell lines Hep-G2 and LU-1 with IC50values of 6.82 and 15.53 mg ml?1, respectively, while free curcumin expresses the activity with IC50values of 7.41 and 18.82 mg ml?1. At the concentration of 40 mg ml?1, NanoGluCur showed anti-tumor promoting effects in reducing tumor size by 59.93% and tumor density by 40.52%, while the percentages caused by pristine curcumin were 41.36% and 29.14%, respectively. These results demonstrated dual effect of 1-3/1-6 ?-glucan encapsulated curcumin nanoparticles: higher water solubility and better in vitroanti-cancer effects compared to free curcumin and 1-3/1-6 ?-glucan, expectedly. This observation can potentially open a new approach in research and manufacture of functional foods from medicinal mushrooms. |
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