| Autor: |
Rouyer-Fessard, P, Garel, M C, Domenget, C, Guetarni, D, Bachir, D, Colonna, P, Beuzard, Y |
| Zdroj: |
Journal of Biological Chemistry; November 1989, Vol. 264 Issue: 32 p19092-19098, 7p |
| Abstrakt: |
The soluble pool of α hemoglobin chains present in blood or bone marrow cells was measured with a new affinity method using a specific probe, β A hemoglobin chain labeled with [3H]N-ethylmaleimide. This pool of soluble α chains was 0.067 ± 0.017% of hemoglobin in blood of normal adult, 0.11 ± 0.03% in heterozygous β thalassemia and ranged from 0.26 to 1.30% in homozygous β thalassemia intermedia. This elevated pool of soluble α chains observed in human β thalassemia intermedia decreased 33-fold from a value of 10% of total hemoglobin in bone marrow cells to 0.3% in the most dense red blood cells. The amount of insoluble α chains was measured by using the polyacrylamide gel electrophoresis in urea and Triton X-100. In β thalassemia intermedia the amount of insoluble α chains was correlated with the decreased spectrin content of red cell membrane and was associated with a decrease in ankyrin and with other abnormalities of the electrophoretic pattern of membrane proteins. The loss and topology of the reactive thiol groups of membrane proteins was determined by using [3H]N-ethylmaleimide added to membrane ghosts prior to urea and Triton X-100 electrophoresis. Spectrin and ankyrin were the major proteins with the most important decrease of thiol groups. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
