| Autor: |
Malaba, Lucie, Smeland, Sigbj, Senoo, Haruki, Norum, Kaare R., Berg, Trond, Blomhoff, Rune, Kindberg, Grete M. |
| Zdroj: |
Journal of Biological Chemistry; June 1995, Vol. 270 Issue: 26 p15686-15692, 7p |
| Abstrakt: |
The intracellular transport and degradation of in vivoendocytosed retinol-binding protein was compared with that of asialo-orosomucoid, a marker for receptor-mediated endocytosis through coated pits. The transport pathways were studied in rat liver cells by means of subcellular fractionation in Nycodenz and sucrose density gradients and by immunoelectron microscopy. Retinol-binding protein and asialo-orosomucoid were labeled by covalent attachment of radioiodinated tyramine cellobiose, an adduct which is incapable of crossing cellular membranes and thus provides a marker for the organelles where the protein has been taken up and degraded. |
| Databáze: |
Supplemental Index |
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