| Autor: |
Li, Xinmin, Lopez-Guisa, Jesus M., Ninan, Nisha, Weiner, Evan J., Rauscher, Frank J., Marmorstein, Ronen |
| Zdroj: |
Journal of Biological Chemistry; October 1997, Vol. 272 Issue: 43 p27324-27329, 6p |
| Abstrakt: |
The BTB/POZ domain defines a conserved region of about 120 residues and has been found in over 40 proteins to date. It is located predominantly at the N terminus of Zn-finger DNA-binding proteins, where it may function as a repression domain, and less frequently in actin-binding and poxvirus-encoded proteins, where it may function as a protein-protein interaction interface. A prototypic human BTB/POZ protein, PLZF (promyelocytic leukemiazinc finger) is fused to RARα (retinoic acid receptor α) in a subset of acute promyelocytic leukemias (APLs), where it acts as a potent oncogene. The exact role of the BTB/POZ domain in protein-protein interactions and/or transcriptional regulation is unknown. We have overexpressed, purified, characterized, and crystallized the BTB/POZ domain from PLZF (PLZF-BTB/POZ). Gel filtration, dynamic light scattering, and equilibrium sedimentation experiments show that PLZF-BTB/POZ forms a homodimer with aKdbelow 200 nm. Differential scanning calorimetry and equilibrium denaturation experiments are consistent with the PLZF-BTB/POZ dimer undergoing a two-state unfolding transition with a Tmof 70.4 °C, and a ΔG of 12.8 ± 0.4 kcal/mol. Circular dichroism shows that the PLZF-BTB/POZ dimer has significant secondary structure including about 457 helix and 207 ॆ-sheet. We have prepared crystals of the PLZF-BTB/POZ that are suitable for a high resolution structure determination using x-ray crystallography. The crystals form in the space group I222 or I212121with a= 38.8, b= 77.7, and c= 85.3 Å and contain 1 protein subunit per asymmetric unit with approximately 407 solvent. Our data support the hypothesis that the BTB/POZ domain mediates a functionally relevant dimerization function in vivo. The crystal structure of the PLZF-BTB/POZ domain will provide a paradigm for understanding the structural basis underlying BTB/POZ domain function. |
| Databáze: |
Supplemental Index |
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