Ethanol treatment selectively decreases neutral amino acid transport in cultured hepatocytes.

Autor: Dorio, R J, Hoek, J B, Rubin, E
Zdroj: Journal of Biological Chemistry; September 1984, Vol. 259 Issue: 18 p11430-11435, 6p
Abstrakt: Primary cultures of adult rat hepatocytes were used to study the effects of 100 mM ethanol on various neutral amino acid transport systems. Ethanol exposure for 24 h selectively decreased amino acid uptake by the A and N systems by 40-70%, but had no significant effect on the ASC and L systems. The decrease in the A system was significant after 3 h of ethanol exposure, and the activity was not affected by the presence or absence of ethanol during the uptake measurements. Kinetic analysis showed that ethanol treatment affected predominantly the high-affinity component of A system activity by decreasing the apparent Vmax without significantly changing the apparent Km. Ethanol treatment did not prevent the cells from increasing A system activity in response to insulin and glucagon, but the magnitude of hormone-stimulated uptake was reduced.
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