| Autor: |
Leroy, Pascale, Dessolin, Sophie, Villageois, Phi, Moon, Byoung Chun, Friedman, Jeffrey M., Ailhaud, Gérard, Dani, Christian |
| Zdroj: |
Journal of Biological Chemistry; February 1996, Vol. 271 Issue: 5 p2365-2368, 4p |
| Abstrakt: |
The product of the recently cloned mouse obese (ob) gene is likely to play an important role in a loop regulating the size of the adipose tissue mass. The hormonal regulation of the obgene could affect adiposity. To investigate this point, the effect of insulin on obgene expression was examined in cells of the 3T3-F442A preadipocyte clonal line. obmRNA is absent from exponentially growing, undifferentiated cells as well as from confluent preadipose cells. Terminal differentiation of preadipose to adipose cells leads to the expression of obmRNA detected by a sensitive and quantitative ribonuclease protection assay. In adipose cells, the level of obmRNA is sensitive to insulin in the nanomolar range of concentrations with an increase from an average of 1 copy to 5-10 copies/cell. The effect of insulin was fully reversible and takes place primarily at a transcriptional level. The obmRNA shows a rapid turnover, with a half-life of approximately 2 h in the absence or presence of insulin. The level of secreted Ob protein is also regulated by insulin. These results indicate that the obgene is expressed in mature fat cells only and support the possibility that insulin is an important regulator of obgene expression. |
| Databáze: |
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