| Autor: |
Bendall, Heather H., Scherer, David C., Edson, Christine R., Ballard, Dean W., Oltz, Eugene M. |
| Zdroj: |
Journal of Biological Chemistry; November 1997, Vol. 272 Issue: 46 p28826-28828, 3p |
| Abstrakt: |
The POU transcription factors Oct-1 and Oct-2 regulate the activity of octamer-dependent promoters, including those that direct transcription from rearranged immunoglobulin genes. Unlike Oct-1, which is constitutively expressed in many cell types, Oct-2 expression is restricted primarily to B lymphocytes and can be induced in precursor B cells by stimulation with bacterial lipopolysaccharide (LPS). However, the precise factors that mediate this induction mechanism remain unknown. In the present study, we monitored Oct-2 expression in cells arrested for the activation of NF-κB, an LPS-responsive member of the Rel transcription factor family. Despite stimulation with LPS, disruption of the NF-κB signaling pathway in precursor B cells led to the loss of inducible Oct-2 DNA binding activity in vitroand the suppression of Oct-2-directed transcription in vivo. This biochemical defect correlated with a specific block to Oct-2 gene expression at the level of transcription, whereas the expression of Oct-1 was unaffected. The finding that Oct-2 is under NF-κB control highlights an important cross-talk mechanism involving two distinct transcription factor families that regulate B lymphocyte function. |
| Databáze: |
Supplemental Index |
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