| Autor: |
Johnson, Robert E., Kovvali, Gopala K., Prakash, Louise, Prakash, Satya |
| Zdroj: |
Journal of Biological Chemistry; March 1996, Vol. 271 Issue: 13 p7285-7288, 4p |
| Abstrakt: |
Defects in DNA mismatch repair result in instability of simple repetitive DNA sequences and elevated levels of spontaneous mutability. The human G/T mismatch binding protein, GTBP/p160, has been suggested to have a role in the repair of base-base and single nucleotide insertion-deletion mismatches. Here we examine the role of the yeast GTBP homolog, MSH6, in mismatch repair. We show that both MSH6and MSH3genes are essential for normal genomic stability. Interestingly, although mutations in either MSH3or MSH6do not cause the extreme microsatellite instability and spontaneous mutability observed in the msh2mutant, yeast cells harboring null mutations in both the MSH3and MSH6genes exhibit microsatellite instability and mutability similar to that in the msh2mutant. Results from epistasis analyses indicate that MSH2functions in mismatch repair in conjunction with MSH3or MSH6and that MSH3and MSH6constitute alternate pathways of MSH2-dependent mismatch repair. |
| Databáze: |
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