Abstrakt: |
We have isolated and characterized a rat gene coding for spot 14 mRNA: a hepatic product induced rapidly by thyroid hormone. This gene is present in a single copy/haploid genome, but encodes two mRNA species differing by 170 nucleotides in length. Through S1 nuclease analyses, the difference was mapped to the 3'-end of the mRNA with one species extending 170 nucleotides beyond the 3'-end of the other mRNA. The putative poly(A) addition signals AUUAAA and AAUAAA are found to precede the sites of polyadenylation in the shorter and longer mRNA, respectively. The mRNA codes for a protein of 150 amino acids with a molecular weight of 17,010. The protein-coding region is located closer to the 5'-end of the mRNA, leaving a relatively long 3'-untranslated region, which contains the only intron of 3,150 base pairs within the gene. At approximately 27 base pairs upstream from the start site of the mRNA, a sequence homologous to the TATA box, TAGAAAT , was found. |