| Autor: |
Saucier, S E, Kandutsch, A A, Taylor, F R, Spencer, T A, Phirwa, S, Gayen, A K |
| Zdroj: |
Journal of Biological Chemistry; November 1985, Vol. 260 Issue: 27 p14571-14579, 9p |
| Abstrakt: |
Biosynthetically tritiated sterols from Chinese hamster lung (Dede) cells were fractionated by high performance liquid chromatography, and fractions were assayed for their ability to repress 3-hydroxy-3-methylglutaryl-CoA reductase in L cell cultures. Most of the activity found was associated with two oxysterols, 24(S),25-epoxycholesterol and 25-hydroxycholesterol. The identities of the two sterols were established by co-chromatography with authentic samples and by isotopic dilution and recrystallization. Only low levels of repressor activity were found in other fractions of the sterol extract. The endogenous concentrations of 24(S),25-epoxycholesterol (7.2 fg/cell) and 25-hydroxycholesterol (1.5 fg/cell) appear to be within the ranges required for the regulation of HMG-CoA reductase. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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