| Autor: |
Mullenbach, G T, Tabrizi, A, Blacher, R W, Steimer, K S |
| Zdroj: |
Journal of Biological Chemistry; January 1986, Vol. 261 Issue: 2 p719-722, 4p |
| Abstrakt: |
A synthetic gene encoding the platelet-derived factor, connective tissue activating peptide-III (CTAP-III) (Castor, C.W., Miller, J.W., and Walz, D.A. (1983) Proc. Natl. Acad. Sci. U. S. A. 80, 765-769), has been expressed and secreted from Saccharomyces cerevisiae by using a yeast expression vector and an alpha-factor leader segment. Mitogenic activity reported for naturally derived CTAP-III has been demonstrated here for recombinant CTAP-III. Active CTAP-III has been purified to apparent homogeneity. Structural studies have confirmed its identity. A general approach for the facile synthesis of genes is presented which has permitted the assembly of the entire structural gene and flanking regions (280 base pairs) from 20 oligomers in a single annealing and ligation reaction pool. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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