| Autor: |
Kempa, Emily E., Galman, James L., Parmeggiani, Fabio, Marshall, James R., Malassis, Julien, Fontenelle, Clement Q., Vendeville, Jean-Baptiste, Linclau, Bruno, Charnock, Simon J., Flitsch, Sabine L., Turner, Nicholas J., Barran, Perdita E. |
| Zdroj: |
JACS Au; 20210101, Issue: Preprints |
| Abstrakt: |
The lack of label-free high-throughput screening technologies presents a major bottleneck in the identification of active and selective biocatalysts, with the number of variants often exceeding the capacity of traditional analytical platforms to assess their activity in a practical time scale. Here, we show the application of direct infusion of biotransformations to the mass spectrometer (DiBT-MS) screening to a variety of enzymes, in different formats, achieving sample throughputs equivalent to ∼40 s per sample. The heat map output allows rapid selection of active enzymes within 96-well plates facilitating identification of industrially relevant biocatalysts. This DiBT-MS screening workflow has been applied to the directed evolution of a phenylalanine ammonia lyase (PAL) as a case study, enhancing its activity toward electron-rich cinnamic acid derivatives which are relevant to lignocellulosic biomass degradation. Additional benefits of the screening platform include the discovery of biocatalysts (kinases, imine reductases) with novel activities and the incorporation of ion mobility technology for the identification of product hits with increased confidence. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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