Diversity of Rickettsiain ticks collected from wild animals in Panama

Autor: Bermúdez, Sergio, Martínez-Mandiche, José, Domínguez, Lillian, Gonzalez, Claudia, Chavarria, Oris, Moreno, Ambar, Góndola, Jessica, Correa, Néstor, Rodríguez, Indra, Castillo, Bernardo, Smith, Diorene, Martínez, Alexander A.
Zdroj: Ticks and Tick-borne Diseases; July 2021, Vol. 12 Issue: 4
Abstrakt: This paper presents new data about Rickettsiaspecies detected in ticks collected from wild animals, using 16S rRNA, gltAand ompA. RickettsiaDNA was found in 66 of 101 ticks. Using EZ BioCloud libraries were produced reads that identified Rickettsia aeschlimannii, and Illumina BaseSpace produced reads of Rickettsia rickettsiigroup, Rickettsia belliigroup, and unclassified Rickettsia. Using gltAand ompAgene-specific primers, R. aeschlimanniicould not be confirmed, but detection of Rickettsia amblyommatiswas achieved in Amblyomma auricularium, Amblyomma geayi, Amblyomma mixtum, and Amblyomma pacae; R. belliifrom Amblyomma dissimile, “CandidatusRickettsia colombianensi” from A. dissimile, Rickettsiaspp. closely related to R. raoultiifrom A. geayi, Rickettsia tamuraefrom A. dissimile, and Rickettsiaendosymbionts of Ixodesfrom Ixodes affinis. There were no databases available specifically for 16S rRNA of Neotropical Rickettsia,highlighting the need to use species primers over only 16S rRNA primers to achieve more accurate interpretations and identifications. These findings increase the number of Rickettsiaspecies detected in Panama and highlight the need to establish isolates to further characterize the nature of Rickettsiain the area.
Databáze: Supplemental Index
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