| Autor: |
Qiao, Wei, Jin, Rui, Luo, Tianpeng, Li, Yafeng, Fan, Guoqing, Luo, Qingming, Yuan, Jing |
| Zdroj: |
Biomedical Optics Express; April 2021, Vol. 12 Issue: 4 p2373-2383, 11p |
| Abstrakt: |
Optical sectioning has been widely employed for inhibiting out-of-focus backgrounds in three-dimensional (3D) imaging of biological samples. However, point scanning imaging or multiple acquisitions for wide-field optical sectioning in epi-illumination microscopy remains time-consuming for large-scale imaging. In this paper, we propose a single-scan optical sectioning method based on the hybrid illumination (HiLo) algorithm with a line-illumination strategy. Our method combines HiLo background inhibition with confocal slit detection. It thereby offers a higher optical sectioning capability than wide-field HiLo and line-confocal imaging without extra modulation and multiple data acquisition. To demonstrate the optical-sectioning capability of our system, we imaged a thin fluorescent plane and different fluorescence-labeled mouse tissue. Our method shows an excellent background inhibition in thick tissue and thus potentially provides an alternative tool for 3D imaging of large-scale biological tissue. |
| Databáze: |
Supplemental Index |
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