| Autor: |
Yanagishita, M, McQuillan, D J |
| Zdroj: |
Journal of Biological Chemistry; October 1989, Vol. 264 Issue: 29 p17551-17558, 8p |
| Abstrakt: |
The structures of cell-associated heparan sulfate (HS) proteoglycans and their interaction with the plasma membrane was studied using rat ovarian granulosa cell culture. HS proteoglycans were either metabolically labeled by incubating cell cultures with [3H] leucine and [35S]sulfate or labeled in plasma membrane preparations with a photoactivatable reagent, 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine (TID), a compound which has been shown to selectively label the hydrophobic membrane-binding domains of several proteins. After purification of HS proteoglycans from the labeled cell cultures or from the labeled membrane preparations by repeated Q-Sepharose ion exchange chromatography in 8 M urea, they were analyzed by Superose 6 gel filtration and octyl-Sepharose chromatography both in 4 Mguanidine HCl. The results indicated that the HS proteoglycans were labeled with 125I and therefore have an intramembranous domain. |
| Databáze: |
Supplemental Index |
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