Autor: |
Chan, Bock G., Cain, John C. |
Zdroj: |
Journal of Chromatography A; January 1966, Vol. 22 Issue: 1 p95-101, 7p |
Abstrakt: |
A simple quantitative procedure for sugar analysis of plant materials by paper chromatography with good reproducibility is described. The alcoholic extract was partially purified by a tertiary solvent system and ion exchange resins and then paper chromatography with the following solvent: water-saturated n-butanol, 95% ethanol and trichloroethylene (6:2:2, v/v). The sugars were selected by a guide strip technique, and eluted from the “cut-out squares” of paper with 3 ml distilled water by a micro-elution tube, here described. Theyb were determined by a micro-colorimetric method. The method permitted 72 determinations to be done at one time. |
Databáze: |
Supplemental Index |
Externí odkaz: |
|