| Autor: |
Xiong, W.J., Zsigmond, E., Gotto, A.M., Lei, K.Y., Chan, L. |
| Zdroj: |
Journal of Biological Chemistry; November 1991, Vol. 266 Issue: 31 p20893-20898, 6p |
| Abstrakt: |
Through its interaction with the low density lipoprotein (LDL) receptor, apolipoprotein (apo) B-100 is a major determinant of LDL metabolism and plasma cholesterol. Its receptor binding ability is conformation-dependent and requires its expression on the right lipoprotein particles. The structural signal that targets apoB-100 to LDL is unknown. We have microinjected a human apoB-100 minigene construct comprising less than 25% of the apoB-100 sequence driven by the natural apoB promoter to produce transgenic mice. The transgene product was expressed at a high level and was present exclusively in the LDL of these animals. Analysis of the responsible sequence (residues 2878-3925 of apoB-100) reveals unique structural features that may be important in its role as an LDL-targeting domain. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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