| Autor: |
Zheng, Feiyun, Wang, Tianmu, Niu, Chengtuo, Zheng, Ruilong, Liu, Chunfeng, Wang, Jinjing, Li, Qi |
| Zdroj: |
Journal of the American Society of Brewing Chemists; October 2020, Vol. 79 Issue: 1 p90-98, 9p |
| Abstrakt: |
AbstractThis study aimed to address the roles of divalent-cation transporter genes of Lactobacillus casei2-9-5 in resisting hop bitter compound iso-α-acid. To determine the underlying mechanisms for Lactobacillus caseistrains to resist iso-α-acid, RNA sequencing was performed for La. casei2-9-5 and W2-9-5, which was an isolate of La. caseiW2-9-5 derivative with a relatively weaker iso-α-acid resistance ability, in 0.012% iso-α-acid solution. Results indicated that its resistance of iso-α-acid was closely related to transmembrane transport, membrane composition and oxidative phosphorylation. Compared with La. caseiW2-9-5, the expressions of the mntA, mntBand mntCgenes were found to be significantly up-regulated in La. casei2-9-5 and confirmed by RT-qPCR analysis. To examine the roles of these genes, recombinant plasmids harboring these genes were constructed and expressed in model bacteria Lactococcus lactisNZ3900. Relative to controls, the recombinant Lc. lactiscontaining mntBand mntCgenes grew better under iso-α-acid pressure. Furthermore, the intracellular Mn2+concentration in La. casei2-9-5 was much higher than that in La. caseiW2-9-5. Together, the data support that manganese transporter genes mntBand mntCare involved in the iso-α-acid resistance ability of La. caseistrains through enhancement of intracellular Mn2+concentrations. |
| Databáze: |
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