Open-source computational framework for studying Drosophilabehavioral phase

Autor: Kostadinov, Boyan, Lee Pettibone, Hannah, Bell, Evardra Valerie, Zhou, Xiaona, Pranevicius, Ausra, Shafer, Orie Thomas, Fernandez, Maria Paz
Zdroj: STAR Protocols; March 2021, Vol. 2 Issue: 1
Abstrakt: This protocol describes a standardized method for analyzing Drosophilabehavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophilagenetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided.
Databáze: Supplemental Index