| Autor: |
Goris, Marga G.A., Hartskeerl, Rudy A. |
| Zdroj: |
Current Protocols in Microbiology; February 2014, Vol. 32 Issue: 1 p12E.5.1-12E.5.18, 11268p |
| Abstrakt: |
The microscopic agglutination test (MAT) is the gold standard for sero‐diagnosis of leptospirosis because of its unsurpassed diagnostic specificity. It uses panels of live leptospires, ideally recent isolates, representing the circulating serovars from the area where the patient became infected. A dilution series of the patient's serum is mixed with a suspension of live leptospires in microtiter plates. After incubating for about 2 hr at 30°C, results are read under the dark‐field microscope. The titer is the last dilution in which ≥50% of the leptospires have remained agglutinated. Seroconversion or ≥4‐fold titer rise in paired sera is consistent with current leptospirosis. The significance of a titer in a single sample depends on the frequency of residual titers due to past infections and cross‐reacting other diseases in the population. Full standardization of the MAT is not possible, but quality assurance can be achieved by participation in the international MAT proficiency testing scheme. Curr. Protoc. Microbiol. 32:12E.5.1‐12E.5.18. © 2014 by John Wiley & Sons, Inc. |
| Databáze: |
Supplemental Index |
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