| Abstrakt: |
Purpose:Campylobacteris one of the most common pathogens that cause food-borne infections worldwide. The aim of this study was to determine the antimicrobial resistance rates and the presence of multiple virulence genes in Campylobacterisolates obtained from humans. Materials and Methods:In this study, 71 Campylobacterisolates obtained from human faecal samples were used. Antimicrobial susceptibility tests were performed through the gradient strip method. The presence of virulence genes was investigated by monoplex and multiplex polymerase chain reaction. Results:The rate of resistance of the 66 Campylobacter jejuniisolates was 12.1% for erythromycin, 40.9% for tetracycline and 68.2% for ciprofloxacin. Only one of five Campylobacter coliisolates was resistant to these three antimicrobial agents. The flaB, pldA, cdtA, cadF, cdtCand ceuEgenes were found in all 66 of the C. jejuniisolates. In the C. jejuniisolates, positivity rates of 92.4% for flaA, 96.7% for cdtB, 98.5% for ciaB, 90.9% for dnaJand 96.7% for racRwere observed. The flaA, flaB, ciaB, cdtAand cdtCgenes were present in all C. coliisolates. Conclusions:It was detected that there is an increase in antimicrobial resistance of Campylobacterstrains in our region, and most of the isolates harbour virulence genes. |
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