| Autor: |
Vilalta, Carles, Sanhueza, Juan Manuel, Murray, Deborah, Johnson, Levi, Pieters, Maria |
| Zdroj: |
The Veterinary Record; October 2019, Vol. 185 Issue: 16 p510-510, 1p |
| Abstrakt: |
Processing fluid (PF) is a sample type composed of fluids obtained from testicles and tails as the product of piglet processing. Mycoplasma hyopneumoniaeis a bacterium that colonises the respiratory tract of pigs and has rarely been detected in tissues outside the respiratory system. No data exist in the literature regarding detection of M hyopneumoniaein PF or its use for herd monitoring of this pathogen. The main goal of this study was to evaluate the feasibility of detecting M hyopneumoniaein PF. Testicles and tails of 21 conveniently selected litters from a commercial sow farm were collected, by litter, and tested for M hyopneumoniaeby real time‐PCR. Daily aggregated processing tissues were collected for a two‐month period to assess the detection of M hyopneumoniaein PF. The comparison in the percentage of positive samples in fluids from testicles (38 per cent, 8/21) or tails (4.8 per cent, 1/21) was significantly different (P=0.023). The percentage of daily aggregated PF with cycle threshold values up to 37 was 52.9 per cent (9/17) and 26.7 per cent (4/15) for December and January, respectively. Overall, these data show detection of M hyopneumoniaein PF for the first time and points at the potential use of this sample for monitoring of this bacterium in breeding farms. |
| Databáze: |
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