Performance of Commercial Mycoplasma hyopneumoniaeSerum Enzyme-Linked Immunosorbent Assays under Experimental and Field Conditions

Autor: Poeta Silva, Ana Paula S., Magtoto, Ronaldo L., Souza Almeida, Henrique M., McDaniel, Aric, Magtoto, Precy D., Derscheid, Rachel J., Merodio, Maria M., Matias Ferreyra, Franco S., Gatto, Igor R. H., Baum, David H., Clavijo, Maria J., Arruda, Bailey L., Zimmerman, Jeffrey J., Giménez-Lirola, Luis G.
Zdroj: Journal of Clinical Microbiology; September 2020, Vol. 58 Issue: 12
Abstrakt: Mycoplasma hyopneumoniaeis an economically significant pathogen of swine. M. hyopneumoniaeserum antibody detection via commercial enzyme-linked immunosorbent assays (ELISAs) is widely used for routine surveillance in commercial swine production systems. Samples from two studies were used to evaluate assay performance. In study 1, 6 commercial M. hyopneumoniaeELISAs were compared using serum samples from 8-week-old cesarean-derived, colostrum-deprived (CDCD) pigs allocated to the following 5 inoculation groups of 10 pigs each: (i) negative control, (ii) Mycoplasma flocculare(strain 27399), (iii) Mycoplasma hyorhinis(strain 38983), (iv) Mycoplasma hyosynoviae(strain 34428), and (v) M. hyopneumoniae(strain 232).
Databáze: Supplemental Index