Identification of race‐specific quantitative trait loci for resistance to Colletotrichum lindemuthianumin an Andean population of common bean

Autor: Mungalu, Handsome, Sansala, Miyoba, Hamabwe, Swivia, Mukuma, Chikoti, Gepts, Paul, Kelly, James D., Kamfwa, Kelvin
Zdroj: Crop Science; November 2020, Vol. 60 Issue: 6 p2843-2856, 14p
Abstrakt: Anthracnose caused by Colletotrichum lindemuthianumis a major disease of common bean (Phaseolus vulgarisL.). Because of the high genetic variability of C. lindemuthianum, development of varieties with durable resistance requires deployment of loci with resistance to a wide range of C. lindemuthianumraces. The objective of the current study was to identify quantitative trait loci (QTL) associated with resistance to nine races of C. lindemuthianumin an Andean population. A total of 150 F4:8recombinant inbred lines (RILs) from Andean parents Solwezi and AO‐1012‐29‐3‐3A were evaluated for resistance to nine races of C. lindemuthianumin a screenhouse at University of Zambia, Lusaka, Zambia. AO‐1012‐29‐3‐3A is resistant to some races of C. lindemuthianum. The population was genotyped with 5,398 SNP markers and composite interval mapping conducted to identify QTL for resistance. A total of 14 QTL for resistance to nine races of C. lindemuthianumwere identified on five chromosomes including Pv02, Pv03, Pv04, Pv07, and Pv10. These 14 QTL included both major and minor QTL with R2values ranging from 7.3% for ANT10.1SAon Pv10 to 79% for ANT2.1UC,SAon Pv02. A majority of the identified QTL were major QTL but a few were minor QTL, suggesting a role for both qualitative and quantitative resistance in the mapping population. The major QTL ANT02.1UC,SAand ANT04.1SA, which in combination provided resistance to all nine races used in the study, colocalize with previously reported major genes and would be ideal targets for pyramiding using marker‐assisted selection to develop varieties with durable resistance to anthracnose.
Databáze: Supplemental Index