| Autor: |
Cao, Wen Xi, Kabelitz, Sarah, Gupta, Meera, Yeung, Eyan, Lin, Sichun, Rammelt, Christiane, Ihling, Christian, Pekovic, Filip, Low, Timothy C.H., Siddiqui, Najeeb U., Cheng, Matthew H.K., Angers, Stephane, Smibert, Craig A., Wühr, Martin, Wahle, Elmar, Lipshitz, Howard D. |
| Zdroj: |
Cell Reports; June 2020, Vol. 31 Issue: 12 |
| Abstrakt: |
In animal embryos, the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents more than half of the protein-coding capacity of Drosophila melanogaster’s genome, and that 2% of this proteome is rapidly degraded during the MZT. Cleared proteins include the post-transcriptional repressors Cup, Trailer hitch (TRAL), Maternal expression at 31B (ME31B), and Smaug (SMG). Although the ubiquitin-proteasome system is necessary for clearance of these repressors, distinct E3 ligase complexes target them: the C-terminal to Lis1 Homology (CTLH) complex targets Cup, TRAL, and ME31B for degradation early in the MZT and the Skp/Cullin/F-box-containing (SCF) complex targets SMG at the end of the MZT. Deleting the C-terminal 233 amino acids of SMG abrogates F-box protein interaction and confers immunity to degradation. Persistent SMG downregulates zygotic re-expression of mRNAs whose maternal contribution is degraded by SMG. Thus, clearance of SMG permits an orderly MZT. |
| Databáze: |
Supplemental Index |
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