| Autor: |
Ye, Chengjin, Chiem, Kevin, Park, Jun-Gyu, Oladunni, Fatai, Platt, Roy Nelson, Anderson, Tim, Almazan, Fernando, de la Torre, Juan Carlos, Martinez-Sobrido, Luis |
| Zdroj: |
mBio; October 2020, Vol. 11 Issue: 5 |
| Abstrakt: |
The pandemic coronavirus (CoV) disease 2019 (COVID-19) caused by severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is a major threat to global human health. To date, there are no approved prophylactics or therapeutics available for COVID-19. Reverse genetics is a powerful approach to understand factors involved in viral pathogenesis, antiviral screening, and vaccine development. In this study, we describe the feasibility of generating recombinant SARS-CoV-2 (rSARS-CoV-2) by transfection of a single bacterial artificial chromosome (BAC). Importantly, rSARS-CoV-2 possesses the same phenotype as the natural isolate in vitroand in vivo. This is the first description of a BAC-based reverse genetics system for SARS-CoV-2 and the first time that an rSARS-CoV-2 isolate has been shown to be phenotypically identical to a natural isolate in a validated animal model of SARS-CoV-2 infection. The BAC-based reverse genetics approach will facilitate the study of SARS-CoV-2 and the development of prophylactics and therapeutics for the treatment of COVID-19. |
| Databáze: |
Supplemental Index |
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