| Autor: |
Li, Yalin, Huang, Qing, Yao, Guohua, Wang, Xiaoyi, Zhang, Fengqiu, Wang, Tao, Shao, Changsheng, Zheng, Xinxin, Jing, Xumiao, Zhou, Huiyue |
| Zdroj: |
Analytical Chemistry; November 2020, Vol. 92 Issue: 21 p14452-14458, 7p |
| Abstrakt: |
The SWI/SNF complex is a highly conserved chromatin remodeling complex and can hydrolyze ATP by its catalytic subunit BRG1 or BRM to reconstruct the chromatin. To investigate whether this ATP-dependent chromatin remodeling could affect the DNA conformation, we therefore regulated (knocked down or overexpressed) BRG1/BRMin the cells and applied Fourier transform infrared (FTIR) spectroscopy to probe DNA conformational changes. As a result, we found that BRG1/BRM was indeed associated with the DNA conformational changes, in which knockdown of BRG1/BRMreduced Z-DNA conformation, while overexpression of BRG1/BRMenhanced Z-DNA conformation. This Z-DNA conformational transformation was also verified using the Z-DNA-binding proteins. Therefore, this work has provided a direct analytical tool to probe Z-DNA transformation upon ATP-dependent chromatin remodeling. |
| Databáze: |
Supplemental Index |
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