| Autor: |
Christova, Iva, Hohenberger, Susanne, Zehetmeier, Carol, Wilske, B. |
| Zdroj: |
Medical Microbiology and Immunology; April 1998, Vol. 186 Issue: 4 p171-175, 5p |
| Abstrakt: |
Lyme borreliosis is the most frequent tick-borne disease in the Northern hemisphere. Here we describe the first isolation of Borrelia burgdorferisensu lato in Bulgaria: the midguts of 47 Ixodes ricinusobtained by flagging from the Central park in Sofia, Bulgaria were cultivated for borreliae in BSK medium. The eight isolates obtained from the ticks and one skin isolate from a Bulgarian patient with erythema migrans were subjected to phenotypic [outer surface protein A (OspA) serotyping] and genotypic analysis (pulsed-field gel electrophoresis typing followed by large restriction fragment pattern analysis after MluI digestion, polymerase chain reaction with 16S rRNA-directed oligonucleotide probes, and restriction fragmenth length polymorphism analysis of rrf-rrl intergenic spacer amplicons). The skin isolate was B. burgdorferisensu stricto, as were four of the tick isolates; the other four tick isolates were B. gariniirepresenting three different OspA serotypes (types 3, 5 and 7). These findings confirm the wide geographic distribution of the different B. garinii-associated OspA serotypes in Europe (shown here for the first time for the Southeastern part of Europe) and of B. burgdorferisensu stricto in the Western hemisphere. These findings have implications for development of diagnostic tests and a borrelia vaccine in Southeastern Europe. |
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