| Autor: |
TEITSMA, C. A., ANGLADE, I., BAILHACHE, T., TUJAGUE, M., TOUTIRAIS, G., DUCOURET, B., KAH, O., TEITSMA, C. A., ANGLADE, I., BAILHACHE, T., TUJAGUE, M., TOUTIRAIS, G., DUCOURET, B., KAH, O. |
| Zdroj: |
Bulletin Français de la Pêche et de la Pisciculture; January 1998, Vol. 1998 Issue: 350 p597-607, 11p |
| Abstrakt: |
The distribution of the glucocorticoid receptor (GR)within the forebrain of rainbow trout has been studied by means of in situ hybridization (ISH) and immunohistochemistry (ICC) using probes and antibodies directed against the AB-domain of the rainbow trout GR (rtGR). Both rtGR mRNA and protein exhibited the same pattern of distribution. A strong labelling was observed in all subdivisions of the preoptic nucleus, in the thalamic region and in the mediobasal hypothalamus (nucleus recessus lateralis, nucleus lateralis tuberis).It was notably found that all magnocellular neurons of the nucleus preopticus pars magnocellularis (NPOmc) were positive with both ISH and ICC. Furthermore, numerous cells of the anterior lobe of the pituitary were positively stained. Double staining studies allowed to identify part of the rtGR expressing cells. At the telencephalic level, it was found that GnRH neurons frequently express rtGR in vitellogenic females. Virtually all tyrosine hydroxylase (TH) cells in the anteroventral part of the preoptic nucleus (NPOav) were rtGR-positive. In this same area, a colocalization between rainbow trout estradiol receptor (rtER) and TH has already been documented, indirectly demonstrating that rtER and rtGR are colocalized. The same holds true for the GTH-II cells of the anterior pituitary which express both types of receptors. These results provide information on the potential sites of cortisol action on the reproductive axis directly at the pituitary level or indirectly on neuroendocrine Systems controllingGTH-II secretion, possibly via GR-ER interactions. |
| Databáze: |
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