| Autor: |
dos Santos Pozza, Jenifer, Voss-Rech, Daiane, dos Santos Lopes, Letícia, Silveira Luiz Vaz, Clarissa |
| Zdroj: |
Poultry Science; May 2020, Vol. 99 Issue: 5 p2690-2695, 6p |
| Abstrakt: |
Chicken is a leading source of thermotolerant Campylobacter, which triggers human foodborne enteritis. This study evaluated thermotolerant Campylobactercontamination of retail chicken in southern Brazil, using qualitative and quantitative analyses. Selective enrichment in Bolton broth for 24 and 48 h after plating onto modified charcoal–cefoperazone–deoxycholate (mCCD) agar and Preston agar was assessed. The combined results of the detection and enumeration methods revealed a frequency of 70% occurrence of thermotolerant Campylobacterin chicken samples. Campylobacterwas enumerated in 60% of the samples, whereas 46% of the samples were positive in the qualitative analysis. Quantitative analysis showed average counts of 3.10 ± 0.15 log10 CFU/sample. Higher numbers of Campylobacter-positive samples were found using 24-h enrichment before plating onto Preston agar (46%) than onto mCCD agar (2%). The majority of isolated strains were identified as Campylobacter jejuni, and Campylobacter coliwas also found but to a lesser extent. Subtyping revealed a clear distinction between strains isolated from different chicken sources. The enriched samples plated onto mCCD agar showed extensive spreading of nonproducing extended-spectrum β-lactamases Proteus mirabilisthat hampered the identification of Campylobactercolonies. P. mirabilisstrains showed resistance to cefoperazone, trimethoprim, and polymyxin B present in broth and plate media used and were inhibited by rifampicin present in Preston agar. The results underline the effect of the spread of contaminant strains on Campylobactercultures, which might be prevented using a recently revised International Organization for Standardization method for qualitative analysis of chicken. |
| Databáze: |
Supplemental Index |
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