| Autor: |
Terui, Yasuhito, Ikeda, Masayuki, Tomizuka, Hiroshi, Kasahara, Tadashi, Ohtsuki, Tetsuya, Uwai, Masaya, Mori, Masaki, Itoh, Takehito, Tanaka, Miyuki, Yamada, Muneo, Shimamura, Seiichi, Ishizaka, Yukihito, Ikeda, Kazuma, Ozawa, Keiya, Miura, Yasusada, Hatake, Kiyohiko |
| Zdroj: |
Blood; October 1998, Vol. 92 Issue: 8 p2672-2680, 9p |
| Abstrakt: |
Tumor cells are eradicated by several systems, including Fas ligand-Fas and tumor necrosis factor (TNF)-tumor necrosis factor receptor (TNFR). In the previous study, we purified an apoptosis-inducing factor (AIF) to homogeneity from a medium conditioned by PDBu-treated HL-60 cells. N-terminal sequence analysis showed that AIF is identical to endothelial interleukin-8 (IL-8). A novel apoptosis system, in which endothelial cells participate via endothelial IL-8 release, is identified here. Human umbilical vein cells (VE cells) produce and secrete IL-8 by stimulation of IL-1? and TNF-?. Endothelial IL-8, which is secreted from VE cells by stimulation of IL-1? and TNF-? , induces apoptosis in myelogenous leukemia cell line K562 cells. Monocyte-derived IL-8 could not induce apoptosis in K562 cells. Moreover, interaction between VE cells and K562 cells induces the release of endothelial IL-8 from VE cells, and the attached K562 cells undergo apoptosis. Moreover, interactions between VE cell and other cell lines, such as HL-60, U937, Jurkat, and Daudi, induce the secretion of endothelial IL-8 and the induction of apoptosis in cell lines. Endothelial IL-8 significantly inhibits tumor growth of intraperitoneal and subcutaneous tumor mass of K562 cells and induces apoptosis in their cells in vivo. Endothelial IL-8 plays an important role in apoptosis involving endothelial cells, which may provide us with a new therapy for hematological malignancies. © 1998 by The American Society of Hematology. |
| Databáze: |
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