| Autor: |
Loughran, TP Jr, Coyle, T, Sherman, MP, Starkebaum, G, Ehrlich, GD, Ruscetti, FW, Poiesz, BJ |
| Zdroj: |
Blood; September 1992, Vol. 80 Issue: 5 p1116-1119, 4p |
| Abstrakt: |
We studied a patient with large granular lymphocyte (LGL) leukemia for evidence of human T-cell leukemia/lymphoma virus (HTLV) infection. Serum from this patient was positive for HTLV-I/II antibodies by enzyme- linked immunosorbent assay (ELISA) and was confirmed positive in Western blot and radioimmunoprecipitation assays. Results of a synthetic peptide-based ELISA showed that the seropositivity was caused by HTLV-II and not HTLV-I infection. Analyses of enzymatic amplification of DNA from bone marrow sections using the polymerase chain reaction (PCR) were positive for HTLV-II specific gag, pol, env, and pX gene sequences. Cloning and sequencing of amplified products showed that the HTLV-II pol and pX sequences in patient DNA differed from the sequences of 17 other HTLV-II isolates examined in our laboratory. HTLV infection may have a role in some patients in the pathogenesis of LGL leukemia. |
| Databáze: |
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| Externí odkaz: |
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